Table 3.
Densitometry readings of protein expression showing adjusted densities (%) relative to parental-control cell line.
| Cell Line | 786-O P | 786-O c1 | 786-O c2 | Caki-1 P | Caki-1 c1 | Caki-1 c2 | A498 P | A498 c1 | A498 c2 |
|---|---|---|---|---|---|---|---|---|---|
| PD-L1 | 100 | 161.3 | 131.3 | 100 | 16.9 | 35.8 | 100 | 1599.3 | 966.4 |
| HIF1α | ND | ND | ND | ND | ND | ND | 100 | 18.3 | 14.8 |
| Cyclin D1 | 100 | 119.9 | 77.5 | 100 | 56.5 | 34.9 | 100 | 122.0 | 338.8 |
| HIF2α | 100 | 45.8 | 24.2 | ND | ND | ND | 100 | 128.5 | 60.8 |
Quantified using ImageJ software (v.1.8.0) (NIH, Bethesda, MD, USA) and expression adjusted according to respective loading controls using modified ImageJ protocol (http://www.lukemiller.org/ImageJ_gel_analysis.pdf, accessed on 10 August 2021). ND; not detected (i.e., raw value less than 50).