Table 5.
IC50 values of parental and sunitinib-resistant clones, c1 and c2, of cell lines 786-O, A498, and Caki-1 cell lines after treatment with either anti-CD274 siRNA or a scramble RNA control (SCR).
| Cell Line | Replicate | P-SCR | P-siRNA | c1-SCR | c1-siRNA | c2-SCR | c2- siRNA |
|---|---|---|---|---|---|---|---|
| 786-O | A | 5.9 | 4.2 | 11.0 | 6.5 | 10.6 | 6.6 |
| B | 3.8 | 4.9 | 11.7 | 9.6 | 8.2 | 6.5 | |
| C | 4.7 | 6.8 | 12.3 | 9.5 | 9.4 | 8.0 | |
| Average | 4.8 | 5.3 | 11.7 | 8.5 * | 9.4 | 7.0 * | |
| A498 | A | 5.5 | 6.31 | 6.7 | 7.7 | 9.6 | 13.3 |
| B | 9.8 | 11.8 | 10.6 | 12.8 | 10.2 | 11.4 | |
| C | 14.1 | 14.8 | 13 | 15.4 | 15.3 | 20.6 | |
| Average | 9.8 | 11 | 10.1 | 12 | 11.7 | 15.1 | |
| Caki-1 | A | 9.3 | 10.8 | 9 | 11.4 | 10 | 11.5 |
| B | 6.4 | 6.7 | 9.5 | 16.1 | 11 | 11.9 | |
| C | 9.7 | 9.2 | 11 | 11.4 | 12 | 12.4 | |
| Average | 8.4 | 8.9 | 9.8 | 13 | 11 | 11.9 |
Values of biological triplicates are indicated by letters A–C. The dose curves used to generate the IC50 values can be found in Figures S6–S8. p-values were calculated by comparing siRNA-treated cells with SCR-treated cells by independent t-test and significance denoted by * p < 0.05.