Figure 2.

IPSC-derived motor neurons from a SOD1^E101G MND patient are more vulnerable to oxidative stress then iPSC-derived motor neurons from a healthy control. IPSC-derived motor neurons from a SOD1^E101G MND patient and a healthy control were matured for three weeks under hypoxic conditions (3% oxygen) and then transferred to a normoxic incubator (21% oxygen) for 38 h in order to induce oxidative stress. Apoptosis was assessed by quantifying the number of caspase 3/7 substrates every 2 h using an Incucyte. Two-way ANOVA revealed that there were significant main effects of time (F(18,144) = 36.53, p < 0.0001), cell line (F(1.8) = 8.677, p = 0.0185), and time × cell line interaction (F(18.112) = 8.830, p < 0.0001). Sidak’s test for multiple comparisons showed that iPSC-derived motor neurons from the SOD1^E101G MND patient showed a significant increase in the number of caspase 3/7 substrates per mm² from 22 h onwards compared to the healthy control. Data presented as mean ± SEM of n = 5 technical replicates; * p < 0.05; # p < 0.01; ^ p < 0.001.