Figure 3.

Effects of miR-29a overexpression on in vivo metabolism of HFD-fed mice. Kinetic changes of O₂ consumption (a), CO₂ production (b), respiration quotient (c), and heat production (d) of animals accommodated individually in Phenomaster metabolic cages at 23 °C for 24 h. miR-29a overexpression reversed HFD-induced O₂ underconsumption (e), CO₂ underproduction (f), respiratory quotient repression (g), and heat underproduction (h), whereas feed (i) and water intake (j) were unaffected. Mean ± S.E. is calculated from 6 mice. * p < 0.05 upon ANOVA test and Bonferroni post hoc test. ND, standard rodent chow; HFD, high-fat diet.