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. 2021 Aug 24;22(17):9114. doi: 10.3390/ijms22179114

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Figure A1 — Gating strategy for cell death measurement with AnnexinV/Propidium iodide staining. Left: Forward-side-scatter to separate single cells from cell clusters. Middle: Separation of cells from cell debris. Right: Separation of the different cell death forms with AnnexinV-FITC and Propidium iodide. Primary necrotic cells: Ax+/Pi++, early apoptotic cells: Ax+/Pi−, late apoptotic cells: Ax+/Pi+. Shown is the gating strategy using an exemplary staining for HSC-4 cells that were evaluated on day 7 of the experiment.