Figure 1.

CD73 expression in the mouse uterus during estrous cycle. (A) Western blot analysis of CD73 in mouse uterus at each stage during estrous cycle (n = 4). β-actin antibody was used for loading control. P, proestrus; E, estrus; M, metestrus; D, diestrus. (B) Relative levels of CD73 in uterus from different stages of estrous cycle. Each value is expressed as a relative value based on metestrus stage of estrous cycle. Data are shown with mean ± SEM. The one-way ANOVA analysis was used to calculate p-value, * p-value < 0.01. (C) Vaginal smear assay was performed to confirm the estrous cycle. NE, nucleated epithelial cell; KE, anucleated keratinized epithelial cell; LK, leukocyte. The black scale bars indicate 50 μm.