Figure 3.

Estrogen effect in CD73 expression in ovariectomized (OVX) model. (A) RT-PCR analysis of CD73 mRNA in the uterus of the OVX mice after E₂ treatment for 0, 1, 6, 12, and 24 h (each group n = 4). Lactoferrin (Lf) gene was used to confirm the response of estrogen after ovariectomy. Ribosomal protein L7 (Rpl7) gene was used as an internal control. (B) Quantitative RT-PCR shows the relative fold change of CD73 expression after estrogen treatment. The relative expression value was based on the value at 0 h after E₂ treatment. (C) Expression of CD73 was analyzed by Western blot analysis in the uterus of the OVX mice after E₂ treatment for 0, 1, 6, 12, and 24 h (each group n = 4). β-actin was used as an internal control. (D) Relative level of CD73 protein intensity in uterus of OVX mice after E₂ treatment. The relative expression value was based on the value at 0 h after E₂ treatment. Data are presented as the mean intensity ± SEM.