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. 2021 Aug 30;22(17):9403. doi: 10.3390/ijms22179403

Figure 4.

Figure 4

The effect of progesterone on CD73 expression in ovariectomized (OVX) mouse uterus. (A) Western blot analysis for relative levels of CD73 in the uterus of the OVX mice after P4 treatment for 0, 3, 6, 12, and 24 h (each group n = 4). β-actin was used as a loading control. (B) Quantitative Western blot analysis showed the relative expression of CD73 in the uterus of the OVX mice after P4 treatment. The one-way ANOVA analysis was used to calculate p-value, * p-value < 0.01. (C) Confocal microscopic images represent the localization and expression level of CD73 (red) and DAPI (blue) in the uterus of the OVX mice treated with P4. Normal rabbit IgG (IgG control) was used as a negative control for a secondary antibody. LE, luminal epithelium; GE, glandular epithelium; S, stroma. The white scale bars indicate 20 μm. (D) Quantitation of the relative levels of CD73 in the uterus of P4-treated OVX mice using imaging software Carl Zeiss ZEN 2012 in luminal epithelium (LE), glandular epithelium (GE), and stroma (S), respectively. The relative expression value was based on the value at 0 h after P4 treatment. Four animals in each group were examined. The one-way ANOVA analysis was used to calculate p-value, * p-value < 0.01.