Figure 2.

Formation of covalent complexes between Iripin-8 and serine proteases. Iripin-8 and selected serine proteases were incubated for 1 h and subsequently analyzed for complex formation by reducing SDS-PAGE. Protein separation differs between (A,B) due to the use of gels with different polyacrylamide contents. Gels show the profile of Iripin-8 serpin alone, various serine proteases alone, and proteases incubated with Iripin-8. Complex formation between fVIIa and Iripin-8 was tested in the presence of tissue factor (TF) at an equimolar concentration. Covalent complexes between Iripin-8 and protease are marked with a red arrow. N: native Iripin-8, C: cleaved Iripin-8.