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. 2021 Aug 31;22(17):9480. doi: 10.3390/ijms22179480

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of RNAi on tick fitness and Borrelia transmission. (A) RNAi of Iripin-8 prolonged the length of I. ricinus nymph feeding compared with the control group (GFP). (B) Weight of fully engorged nymphs with Iripin-8 knockdown was not different from the control group (GFP). (C) Presence of B. afzelii spirochetes in mouse tissues after infestation with infected I. ricinus nymphs. There were no significant differences between Iripin-8 knockdown and GFP control groups in any of the tested tissues. *** p ≤ 0.001; n.s., not significant.