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. 2021 Aug 26;22(17):9216. doi: 10.3390/ijms22179216

Figure 1.

Figure 1

Characterization of layer-by-layer (LbL) synthesis of siRNA-AuNP nanoformulations with CS or PLA as outer layer. (A) Schematic representation of the LbL nanoformulations with either CS (AuNPs@CS) or PLA (AuNPs@PLA) as the 3rd and final layer. (B) UV–vis spectra upon sequential adsorption of the different layers in the LbL synthesis. (C) Particle size and zeta potential measured by DLS after sequential adsorption of the different layers in the LbL synthesis. (D) Agarose gel retardation assay of nanoformulations upon addition of each layer. After centrifugation, the supernatant was collected and the pellet of nanoformulations was redispersed in water or mixed with 20 mg/mL sodium dodecyl sulfate (SDS) for dissociation of siRNA from the nanoparticles. (E) Percentage of siRNA released from AuNPs@CS and AuNPs@PLA in pH 7.4 HEPES buffer at 37 °C as a function of time. (F) Size and zeta potential analysis in ddi water as a function of time for AuNPs@CS and AuNPs@PLA. Data are represented as mean ± the standard deviation of the mean for a minimum of three independent experiments (ns = not significant).