Figure 5.

Comparison of the interaction of the strongest binding aptamer, RNA 54 (left), and the least strong, RNA 10 (right), to purified exosomes. (A) Change in the fluorescence of exosome-bound CTB-555 and fDiI during titration with RNA aptamer 54 (left) and RNA aptamer 11 (right). F_max is the maximal CTB-555 and fDiI fluorescence obtained before titration with RNA acceptor. ΔF is the difference between measured fluorescence and F_max. (B) Reciprocal of CTB-555 and fDiI fluorescence change during titration with RNA aptamer 54 (in left) and aptamer 10 (right). The regression line equation and the coefficient of determination (R²) are noted above the calculated K_D value in the top-right corner of the chart.