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Time course of the tyrosinase-catalyzed oxidation of 3’-hydroxy-EQ 2 in the presence of BSA. (a) UV/visible spectral changes of 3’-hydroxy-EQ 2 in the presence of 3 mol eq. BSA (containing 0.90 mol eq. SH group) at pH 6.8; (b) UV/visible spectral changes of 3’-hydroxy-EQ 2 in the presence of 3 mol eq. NEM-modified BSA at pH 6.8. Data were obtained from single experiments, but reproducibility was confirmed for each experiment.
