Figure 4.

Classical pathway proteins C1s and C4 accumulated in NaIO₃ damaged the retinas. (A) C1s immunoreactivity (green) was localised (white arrows) to the ONL at three days after the NaIO₃ treatment. At ten days, no specific C1s staining remained visible. DAPI staining (blue) delineated cell nuclei. (B) C1s protein levels were not changed in the Simple Western^TM three days after the NaIO₃ treatment. (C) C4 (green) was observed in the nerve fibre layer (NFL) ten days following the NaIO₃ treatment. In contrast, no distinct C4 staining was visible at day 3. Scale bar: 50 µm. (D) C4 cleavage products C4d (47 kDa) and iC4b (61 kDa) were increased at day 3 after the NaIO₃ treatment in all samples compared to the untreated control using Simple Western^TM technology. (B,D) Intensity of the signals was measured using ImageJ software. * 0.01 < p < 0.05 (one-way ANOVA and Tukey’s multiple comparisons test). The full blots are shown in Supplementary Figure S3C,D.