Figure 5.

Alternative pathway component CFB and CFH protein levels were increased after the NaIO₃ treatment. (A) The CFB (~110 kDa) protein levels were increased at day 3 after the NaIO₃ treatment in all the samples compared to an untreated control using Simple Western^TM technology for detection. (B) Complement inhibitor CFH (~162 kDa) accumulated in the neurosensory retina of NaIO₃-treated mice compared to the control. (C) Retinal degeneration using NaIO₃ resulted in CFH deposition in the photoreceptor outer segment layer shown in immunohistochemical stainings. An overlapping with microglia marker IBA-1 was observed. (A,B) GAPDH was used as a housekeeping protein. The intensity of the protein signal was measured using ImageJ software. ** 0.001 < p < 0.01 (unpaired, nonparametric Mann–Whitney t-test). The full blots are shown in Supplementary Figure S3E,F.