Figure 6.

NaIO₃ increased the retinal transcription of the chemokines and cytokines. RNA-seq (day 3, green) and RT-qPCR analysis (day 3, red; day 10, blue) of the ccl-2, 3, 5, il-1β and tgf-β expression showed a significant upregulation after the NaIO₃ treatment. Il-33 was not significantly upregulated, whereas vegf-a was decreased on the mRNA level at day 3 following the treatment. The expression was normalised to the overall gene set (RNA-seq) or idh3b reference mRNA (RT-qPCR), and the fold change in the expression (compared to the untreated control, line at y = 0) was calculated on the logarithm to base 2. * 0.01 < p < 0.05, ** 0.001 < p < 0.01, *** 0.0001 < p < 0.001 and **** p < 0.0001 (ordinary one-way ANOVA and Tukey’s multiple comparisons test).