. 2021 Sep 6;13(17):4492. doi: 10.3390/cancers13174492

Table 2.

Preclinical evaluations of therapeutic approaches based on miRNA in PCa.

TargetedmiRNA	Therapy Type	Delivery Technology/Vector	Cell Lines	In Vivo	Delivery Route In Vivo	Results	References
miR-16	Mimic	Atelocollagen	22Rv1 Du145 PPC-1 PC-3M-luc	PC-3M-luc	i.v	Local tumor growth inhibition in bone and downregulation of CDK1 and CDK2	[111]
miR-21	Anti-miR	Modified locked nucleic acid (LNA), poly nucleic acid (PNA)	DU145 PC3	DU145	i.v	Inhibition of PTEN and cell proliferation	[106]
miR-21	Anti-miR	Modified locked nucleic acid (LNA), poly nucleic acid (PNA)	DU145 PC3	DU145	i.v	Reduced bone metastasis	[106]
miR-34a	Mimic	Chitosan nanoparticles	PC3	PC3	i.v	Induction of autophagy and apoptosis by downregulation of MET and Axl and c-Myc	[112]
miR-34a	Mimic	Chitosan nanoparticles	PC3	PC3	i.v	Inhibition of tumor growth and metastasis preservation of bone integrity in vivo	[112]
miR-124	Mimic	PEI functionalized polyhydroxybutyratenanoparticles (PHB-PEI)	PC3	-	-	Inhibition of proliferation, motility, and colony formation, through CPT1A modulation	[113]
miR-133a-3p	Mimic	Cationic-lipid transfection (Lipofectamine™ 3000)	VCaP C4-2B PC3	PC3	i.v	Activation of PI3K/AKT pathway and downregulation of EGFR, FGFR1, IGFR1	[114]
miR-133a-3p	Mimic	Cationic-lipid transfection (Lipofectamine™ 3000)	VCaP C4-2B PC3	PC3	i.v	Reduced tumor spread and bone metastasis in vivo	[114]
miR-141 & miR-375	Mimic	cationic polymer polyethyleneimine (PEI) nanoparticles	LNCaP PC3 DU145	PC3	i.p	Reduced cell proliferation and increased apoptosis	[115]
miR-141 & miR-375	Mimic	cationic polymer polyethyleneimine (PEI) nanoparticles	LNCaP PC3 DU145	PC3	i.p	Tumor growth inhibition and up-regulation of SEC23A and PHLPP1 in vivo	[115]
miR-143	Mimic	cationic polymer polyethyleneimine (PEI) nanoparticles	PC3 DU145	PC3	i.p	Tumor growth inhibition and decreased UPAR protein levels	[116]
miR-145	Mimic	PEI-modified magnetic nanoparticles with R11 peptide coating (R11-SSPEI)	PC3 LNCaP	PC3	i.p	Tumor growth inhibition and prolonged survival in vivo	[59]
miR-146a + cetuximab	Mimic	miR-146a and cetuximab-loaded nanocomplexes (PEI-PBA-miR-146a and PEI-DMA-cetuximab)	DU145	DU145		EGFR silencing and reduced cell growth, invasion, and migration in vitro	[117]
miR-146a + cetuximab	Mimic		DU145	DU145		Tumor growth inhibition by enhanced chemosensitivity in vivo	[117]
miR-155-5p	Mimic	Cationic-lipid transfection (Lipofectamine™ 2000)	PC3	-	-	Decreased cell migration and invasion by up-regulation of vimentin, N-cadherin, E-cadherin, β-catenin, MMP3 and MMP9	[118]
miR-155-5p	Mimic	Cationic-lipid transfection (Lipofectamine™ 2000)	PC3	-	-	Inhibition of SPOCK1	[118]
miR-205 + docetaxel	Mimic	Magnetic nanoparticle-based platform with iron oxide core (PEI-PEG)	C4-2 PC3	-	-	Enhanced chemosensitivity and apoptosis	[119]
miR-217 + miR-181b-5p + taxan	Mimic	Transfection (DharmaFECT-1)	PC3 DU145	-	-	Enhanced chemosensitivity and apoptosis	[120]
miR-217 + miR-181b-5p + taxan	Mimic	Transfection (DharmaFECT-1)	PC3 DU145	-	-	Downregulation of more than a thousand different transcripts, which were enriched for genes with cell proliferation and focal adhesion functions	[120]