Figure 3.

Reactive oxygen species generation in MCF-7 cells after 24 h treatment with silver nanoparticles in three different concentrations—50, 25, and 12.5 µg/cm³. (A) The generation of superoxide measured with a dihydroethidium (DHE) fluorescent probe. The amount of generated hydrogen peroxide (B) was measured with a dihydrorhodamine 123 (DHR123) fluorescent probe. Finally, the overall amount of ROS (C) was measured with a 2′,7′-dichlorodihydrofluorescein diacetate (H₂DCFDA) fluorescent probe. The presented data were normalized to cell viability and compared with the control.