Table 1.
Antibodies used for protein detection via the Western blot method; r—rabbit protein, m—mouse protein.
| Antigen | Antibody Clone 1° (Manufacturer) | Antibody Isotype | Antibody 2° |
|---|---|---|---|
| β-catenin | D10A8 (Cell Signaling) | Rabbit IgG1 | Goat anti-rIgG1 conjugate with HRP |
| E-cadherin | 24E10 (Cell Signaling) | Rabbit IgG1 | Goat anti-rIgG1 conjugate with HRP |
| Snail | C15D3 (Cell Signaling) | Rabbit IgG1 | Goat anti-rIgG1 conjugate with HRP |
| ZO-1 | D7D12 (Cell Signaling) | Rabbit IgG1 | Goat anti-rIgG1 conjugate with HRP |
| ZEB1 | D80D3 (Cell Signaling) | Rabbit IgG1 | Goat anti-rIgG1 conjugate with HRP |
| Claudin-1 | D5H1D (Cell Signaling) | Rabbit IgG1 | Goat anti-rIgG1 conjugate with HRP |
| β-actin | AC-74 (Sigma Aldrich) | Mouse IgG1 | Rabbit anti-mIgG1 conjugate with HRP |
| MTA3 | 428C2a (Santa Cruz Biotechnology) | Mouse IgG1 | Rabbit anti-mIgG1 conjugate with HRP |