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Induction of T helper 1 and cytotoxic T 1 (Tc1) immune responses in the mLN by intranasal administration with ECF. C57BL/6 mice were i.n. administered with 50 mg/kg ECF twice at a 3-day interval. (A) Intracellular producing levels of IFN-γ and TNF-α in mLN CD4 and CD8 T cells were measured. (B) Mean percentage of IFN-γ-producing (left panel) and TNF-α-producing (right panel) CD4 and CD8 T cells (n = 6, two-way analysis of variance, ** p < 0.01).
