Figure 3.

Characterization of the stable eys^Δexon40-44 zebrafish line. (A) Schematic representation of exon excision approach (upper panel). Sanger sequencing confirmed the presence of the anticipated exons 40-44 excision in injected embryos (1 day post fertilization (dpf)); lower panel). (B) PCR analysis identified germline transmission in 8 out of 8 pools of 1 dpf embryos obtained by breeding an injected adult with a wild-type animal. PCR (+): positive PCR control, PCR (-): negative PCR control.