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. 2021 Aug 25;22(17):9154. doi: 10.3390/ijms22179154

Figure 4.

Figure 4

Transcript analysis of eysΔexon40-44 mRNA. (A) RT-PCR analysis revealed the absence of eys exons 40-44 in eysΔexon40-44 larvae (5 days post fertilization (dpf)), in the absence of any clear alternatively spliced eys transcripts. (B) Sanger sequencing of the eysΔexon40-44 amplicon confirmed the absence of exons 40-44 from the transcript. (C) Long-range PCR analysis did not reveal any additional and unwanted amplicons derived from CRISPR/Cas9-induced alternative splicing of eys transcripts. PCR (-): negative PCR control.