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. 2021 Aug 25;22(17):9154. doi: 10.3390/ijms22179154

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunohistochemistry on retinal sections of wild-type, eys^KO and eys^Δexon40-44 zebrafish. Retinal cryosections of wild-type, eys^KO and eys^Δexon40-44 larvae (5 days post fertilization (dpf)) stained with antibodies directed against Eys (red) and centrin (green). Nuclei are counterstained with DAPI (blue). In eys^KO and in eys^Δex40-44 zebrafish retinas no Eys signal was detected, whereas in the retinas of wild-type larvae Eys was present adjacent to the centrin immunoreactivity. Scale bar: 10 μm. OS: outer segment, CC: connecting cilium, IS: inner segment, ONL: outer nuclear layer.