Skip to main content
. 2021 Aug 26;22(17):9263. doi: 10.3390/ijms22179263

Figure 2.

Figure 2

BGP-15 mitigates high Pi-induced ECM calcium deposition and Pi uptake of VSMCs in NG and HG conditions. VSMCs were cultured in calcification medium containing 3 mmol/L inorganic Pi under normal glucose (5.5 mM glucose; NG) and high glucose (11 mM; HG) conditions in the presence or absence of BGP-15 (15–100 µM BGP-15 in NG and 25–200 µM in HG condition) for 10 days. (A) Representative Alizarin Red staining is shown from three independent experiments. (B) Calcium content of solubilized ECM in NG and (C) HG conditions is presented. Data are expressed as mean ± SEM of three independent experiments. (D) Intracellular Pi levels in NG and (E) HG conditions are presented as mean ± SEM of three independent experiments. Statistical analysis was performed by the one-way ANOVA test followed by Bonferroni correction. A value of p < 0.05 was considered significant. ns: Non-significant; * p < 0.05, ** p < 0.01, *** p < 0.001.