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. 2021 Aug 26;22(17):9263. doi: 10.3390/ijms22179263

Figure 5.

Figure 5

BGP-15 decreases KLF-5 expression and nuclear translocation induced by high Pi in NG and HG conditions. VSMCs were treated as in Figure 2. Expression of KLF-5 was analyzed by RT-qPCR and immunoblot (A–F). Relative expression of KLF-5 mRNA (A) and protein (B,C) in NG condition ((B): Protein from nuclear extracts normalized to Lamin B1; (C): Cytosolic fractions normalized to GAPDH). Relative expression of KLF-5 mRNA (D) and protein (E,F) in HG condition ((E): Protein from nuclear extracts normalized to Lamin B1; (F): Cytosolic fractions normalized to GAPDH). Data are presented as mean ± SEM of three independent experiments. Relative mRNA expression was normalized to RNA45S5. Statistical analysis was performed by the one-way ANOVA test followed by Bonferroni correction. A value of p < 0.05 was considered significant. * p < 0.05, ** p < 0.01, *** p < 0.001.