Figure 7.

BGP-15 decreases BMP-2 expression induced by high Pi in NG and HG conditions. VSMCs were treated as in Figure 2. Expression of BMP-2 was analyzed by RT-qPCR and immunoblot (A–D). Relative expression of BMP-2 mRNA (A) and protein (B) in both NG and HG conditions ((C): mRNA; (D): protein) is shown as mean ± SEM of three independent experiments. Relative mRNA expression was normalized to RNA45S5, while protein expression to GAPDH. Statistical analysis was performed by the one-way ANOVA test followed by Bonferroni correction. A value of p < 0.05 was considered significant. * p < 0.05, ** p < 0.01, *** p < 0.001.