Figure 11.

BGP-15 decreases microcalcification, the loss of MGP, and the induction of Annexin A2 induced by high Pi in NG and HG conditions. (A) VSMCs were treated as in Figure 2 using 50 µM BGP-15 under NG and 150 µM under HG conditions for 10 days, then plated to glass coverslips. Calcium deposition was visualized by OsteoSense 680. Nuclei were counterstanied with Hoechst staining. Bar represents 50 µm. (B) BGP-15 attenuates the loss of MGP induced by high Pi in NG and HG conditions. VSMCs were treated as in Figure 2 using 50 µM BGP-15 under NG and 150 µM under HG conditions for 10 days, EVs were analyzed by immunoblotting. (C) BGP-15 decreases Annexin A2 expression induced by high Pi in NG and HG conditions. VSMCs were treated as in Figure 2 using 50 µM BGP-15 under NG and 150 µM under HG conditions for 10 days, EVs were analyzed by immunoblotting.