Figure 1.

Results of phenotypic experiments. (A) Growth kinetics in LB broth and (B) statistical comparison of area under the curve (AUC) of growth curves. The results are given as mean values and standard deviation of AUCs. (C) Survival in 50% human serum. The results are given as mean values and standard deviation of log₂ fold change of CFU/mL after 4 h of incubation in serum. (D) Siderophore production ability (n = 3). The results are given as mean values of siderophore production unit and standard deviation. (E) Determination of mucoid phenotype using sedimentation assay (n = 3). The results are given as mean ratios of OD₆₀₀ of supernatant after centrifugation at 1000× g for 5 min and OD₆₀₀ of the overnight culture and standard deviation. For all results the clinical isolates were compared to PBIO1953 (K. pneumoniae, ST307) and hvKP1 (archetypical hypervirulent K. pneumoniae) in variance analyses (one-way ANOVA with Dunnett’s multiple comparison post-hoc test). The significance level (p value) is as follows: ns not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.