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. 2021 Aug 27;22(17):9275. doi: 10.3390/ijms22179275

Figure 4.

Figure 4

WRKY33 regulates the low Pi signaling that intersects with Fe signaling. (A) The growth phenotype of wild-type (WT) and mutant seedlings. Four-day-old seedings were transferred to +Pi +Fe, −Pi+Fe, +Pi−Fe, and −Pi−Fe medium for six days. (B) The statistical analysis of the primary root length as indicated in (A). Data are means ± SD from three independent experiments (n = 15; n represents the number of samples). (C) Root hair growth in WT, wrky33-2, and COM-1 and COM-2 seedlings. Four-day-old seedings were transferred to +Pi+Fe, –Pi+Fe, +Pi−Fe, and −Pi−Fe medium for three days. The experiments were repeated three times with similar results. Bar = 200 μm. (D,E) Root hair number (D) and root hair length (E) as indicated in (C). The hair density and length measured within a 1 mm zone in the root starting from the root tip. Data are means ± SD from three independent experiments (n = 15). Asterisks in (B,D,E) indicate a significant difference from the WT (Tukey’s test; **, p < 0.01).