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. 2021 Aug 27;22(17):9275. doi: 10.3390/ijms22179275

Figure 5.

Figure 5

WRKY33 interacts with ALMT1 to regulate Pi deficiency responses. (A,B) The RT-qPCR assay of ALMT1 (A) and STOP1 (B) expression in wild-type (WT) and wrky33-2 mutant plants. Four-day-old seedlings were grown on a Pi-sufficient (+Pi) or a Pi-deficient (−Pi) medium for three days. The whole plants were excised for RNA extraction and qRT-PCR analysis. ACTIN2 was used as the internal reference gene. (C) The growth phenotype of WT and mutants on +Pi and −Pi media. Four-day-old seedings were transferred to a Pi-sufficient (+Pi) or a Pi-deficient (−Pi) medium for six days. (D) The statistical analysis of the primary root length as indicated in (C). Data are means ± SD from three independent experiments (n = 15; n represents the number of samples). (E) Root hair growth in WT, wrky33-2, complementation lines, wrky33-2 almt1, wrky332 stop1, almt1, and stop1 seedlings. Four-day-old seedings were transferred to a Pi-sufficient (+Pi) or a Pi-deficient (−Pi) medium for three days. Bar = 200 μm. (F,G) The root hair number (F) and the root hair length (G) as indicated in (E). The hair density and length measured within a 1 mm zone in the root starting from the root tip. Data are means ± SD from three independent experiments (n = 15; n represents the number of samples). Asterisks in (A,B,D,F,G) indicate a significant difference from the WT (Tukey’s test; **, p < 0.01).