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. 2021 Aug 25;22(17):9195. doi: 10.3390/ijms22179195

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A,B) Comparison of concentrations of eicosanoids released from transformed HBEC-12KT-B1 and non-transformed HBEC-12KT cells. AA and the evaluated eicosanoids were isolated from cell culture media by solid-phase extraction and then analyzed by HPLC-MS/MS. The results represent means ± standard deviations obtained in three independent experiments in triplicates. The symbols ‘*’ and ‘**’ denote a significant difference (p < 0.05 and p < 0.01, respectively). Abbreviations: AA, arachidonic acid; HETE, hydroxyeicosatetraenoic acid; HODE, hydroxyoctadecadienoic acid; PG, prostaglandin.