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Met receptor activation by wild-type and mutant NK1 (A) and HGF (B). EHMES-1 cells were treated with recombinant protein for 10 min and Met Tyr1234/1235 phosphorylation was determined by cell-based ELISA. The phosphorylation level induced by 1 nM wild-type HGF was set to 1.0. Data represent mean ± s.e.m [n = 3, independent experiments, two-way analysis of variance (ANOVA)].
