Figure 3.

Phosphorylation profiles of Met signaling pathway by wild-type and mutant NK1 or HGF proteins. Serum-starved EHMES-1 cells were stimulated with the recombinant proteins and 5% fetal bovine serum for 10 min. Phosphorylated individual residues of Met receptors, Gab1, Akt, and Erk were detected by Western blotting. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was monitored to ensure equal loading. This experiment was performed once.