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. 2021 Aug 26;22(17):9232. doi: 10.3390/ijms22179232

Figure 4.

Figure 4

NF-κB activation upon illumination of opto-TLR4 PANC-1. (A) Western blot analysis with antibodies against phospho-p65, p65 and (B) phosphor-ERK1/2 and ERK1/2 30 min after light or LPS (100 ng/mL) stimulation. Vinculin was used as the reference gene. (C) Nuclear localisation of p65 after LPS (100 ng/mL) or light (470 nm, 7.5 min) stimulation compared to untreated cells or cells treated with PAR (3 µM). (D) Relative Fluorescence units of nuclear localisation of p65 [nucleus/cytoplasm]. Post-ANOVA multiple comparisons relative to the control were performed using Dunnett’s test. Error bar = SD. n = 5; * p < 0.05; *** p < 0.001.