FIG. 7.

Total splicing and total PA of MXSVL substrates containing LSMs in the DSEs of the PA signal. Mutations DM1 to DM5 (Fig. 6) were introduced into the MXSVL substrate (see the DSM LSMs substrates in Fig. 2) and analyzed for in vitro splicing and PA under coupling conditions (1.5 mM Mg²⁺). Total splicing and total PA were quantitated as a percentage of processing obtained with the WT Sp/PA substrate (WT). The error of the data was ±2 to 4%.