Tumor responses and overall survival in naïve and immunized (i) mice treated with IT saline (CTRL) or CVA21. (A) Tumor growth measured in CTRL (n = 10), CTRL (i) (n = 10), CVA21 (n = 10), and CVA21 (i) (n = 10) treated mice over 70 days. Responses are classified as progressive disease (PD), partial response (PR), or complete response (CR) according to RECIST 1.1 criteria. (B) Survival of CTRL (n = 10), CTRL (i) (n = 10), CVA21 (n = 10), and CVA21 (i) (n = 10) over 70 days. Survival distributions were compared using a Log-rank (Mantel-Cox) test. p values < 0.05 were considered significant (p < 0.05 *, < 0.001 ***, < 0.0001 ****).