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. 2021 Sep 3;13(17):4446. doi: 10.3390/cancers13174446

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) Comparative scatterplot demonstrating the enumeration of CTCs using the RUBYchip™, in light grey versus the CellSearch^® system, in dark grey, for all the 30 samples analysed. (b) Representative images of captured cells using both technologies. CellSearch^® on the bottom, and RUBYchip™ on the top. RUBYchip™ technology allows to obtain high-resolution images, and image quality is clearly superior when compared to CellSearch^®. Isolated cells trapped between pillars of the RUBYchip™ were stained with Anti-Cytokeratin pan-FITC, Cyanine5 Anti-Human CD45, DyLight 550 Anti-ErbB2/HER2 and the nuclear marker DAPI.