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Immunofluorescent staining characteristics for identifying CTCs.
| Characteristic | Cell Classification |
|---|---|
| Irregular shape or dark outline | Debris |
| DAPI+/CK+/HER2−/CD45− | CTC |
| DAPI+/CK+/HER2+/CD45− | |
| DAPI+/CK−/HER2−/CD45+ | WBC |
| DAPI+/CK−/HER2+/CD45+ | |
| DAPI+/CK+/HER2−/CD45+ | Favour WBC |
| DAPI+/CK+/HER2+/CD45+ | |
| DAPI+/CK−/HER2+/CD45+ |
