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. 2021 Aug 27;22(17):9279. doi: 10.3390/ijms22179279

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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In vitro functional characterization of single CTC and CTC-cluster models. (a) Migration of single and cluster models derived from MDA-MB-231^eGFP-Luc (n = 6) and MCF7^eGFP-Luc (n = 4) cell lines. Data are expressed as the fold change of migrated cells relative to the migration observed in the single cell population; (b) Number of invaded MDA-MB-231^eGFP-Luc (n = 3) and MCF7^eGFP-Luc (n = 2) cells per field of view (FOV); (c) In vitro adhesion to endothelium of MDA-MB-231^eGFP-Luc (n = 4) and MCF7^eGFP-Luc (n = 2) cells. Data are expressed as the average of adhered cells per well; (d) Average number of colonies generated by MDA-MB-231^eGFP-Luc (n = 3) and MCF7^eGFP-Luc (n = 3) cells in the soft agar colony formation assays. Data are represented as the average number of colonies. * p < 0.05, ** p < 0.01, *** p < 0.001.