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. 2021 Aug 24;22(17):9113. doi: 10.3390/ijms22179113

Figure 6.

Figure 6

INHBA regulates follicular atresia and GC apoptosis and is targeted by miR-214-5p/miR-7144-3p/miR-9830-5p. (A) differential expression of INHBA mRNA in HF and AF detected by qRT-PCR (n = 7); (B) detection of the interference efficiency of si-INHBA; (C) effect of si-INHBA transfection on pGCs apoptosis; (D–F) miRNAs bind to INHBA 3′-UTR, as confirmed by the luciferase reporter assay; (G) expression level of INHBA after miR-214-5p, miR-7144-3p, and miR-9830-5p overexpression in GCs detected by qRT-PCR; (H) protein expression level of INHBA after miR-214-5p, miR-7144-3p, and miR-9830-5p overexpression in the culture of GCs, detected by ELISA. Data are expressed as the mean ± SEM of three experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.