Figure 2.

OIP5 promotes oncogenic processes of ACHN cells in vitro and in vivo. (A) ACHN empty vector (EV) and OIP5 stable lines. Western blot was carried out using anti-OIP5 and Actin antibodies. OIP5 expression was normalized to Actin and presented at fold changes to OIP5 expression in EV cells. (B) ACHN EV and ACHN OIP5 cells were seeded in 6-well plate at 10⁵ cell/well; cell numbers were recorded at the indicated days. Experiments were repeated three times; means ± SDs are graphed. Statistical analysis was performed using 2-way ANOVA. ***: p < 0.001 between the two curves. (C) The indicated cells were seeded at the indicated number in 6-well plates. Colonies were formed following 2 weeks culture. Experiments were repeated three times; means ± SDs are graphed. **: p < 0.01 compared to the respective EV by Student’s t-test (2-way). (D,E) Invasion and soft agar assays were repeated 3 times; means ± SDs are graphed. **: p < 0.01, ***: p < 0.001 compared to the respective EV control by Student’s t-test (2-way). (F,G) Xenografts were produced in NOS/SCID mice (5 mice per group) using ACHN EV cells and ACHN OIP5 cells. Means ± SEM (standard error of the mean) are graphed; ***: p < 0.001 between the two curved by two-way ANOVA (F). Kaplan-Meier curve; statistical analysis was performed using logrank test (G).