Table 1.
Forward Primers | Backward Primers | |
---|---|---|
Hdac4 | CAGGAGATGCTGGCCATGAA | GCACTCTCTTTGCCCTTCTC |
Hdac5 | GCTTCTTTGGACCAGAGTTCC | CATCTCAGTGGGGATGTTGG |
Sirt1 | CTTCAGTGTCATGGTTCCTT | ACCGAGGAACTACCTGATTA |
Myog | GTCCCAACCCAGGAGATCATT | AGTTGGGCATGGTTTCGTCT |
36B4 | CGCTTTCTGGAGGGTGTCCGC | TGCCAGGACGCGCTTGTACC |
The 2−ΔΔCt method was used to analyze the relative fold changes in gene expression level normalized against 36B4 expression.