Figure 1.

Anxa2 regulated the expression of VEGFs. HepG2 cells were treated with three different Anxa2-shRNAs. (A) Protein level and phosphorylation status of STAT3 and Anxa2 were determined by immuno-blotting. (B) The activation of STAT3 was determined via reporter gene assay. (C) The expression of STAT3 target genes was determined by qRT-PCR. (D) The protein level of four VEGFs was determined by immuno-blotting. (E) The concentration of four VEGFs in cell-free supernatant was determined by ELISA. All experiments were performed with triple replicant. Significance analysis was shown as ** (p < 0.01) and *** (p < 0.001).