Figure 2.

Anxa2 in HepG2 cells promoted proliferation and migration of co-cultured HUVECs. (A) Three co-culture arrangements were shown as schematic plots. (B,C) Colony formation of HUVECs were performed with cell-free supernatant from HepG2 cells with Anxa2 knock-down or not. The cell amount of each colony was shown as superimposed symbols with connecting lines (B), and the overall status of colony formation was shown as images after crystal violet staining (C). (D,E) EdU-labeling was utilized for new DNA synthesis detection in HUVECs co-cultured with HepG2 cells. EdU-positive cells were shown as florescent images (D), and EdU-positive ratio was shown as histogram (E,F) Migrated HUVECs co-cultured with HepG2 cells were shown as images after crystal violet staining. All experiments were performed with triple replicant. Significance analysis was shown as *** (p < 0.001). Scale bar presented 20 μm.