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. 2021 Sep 4;22(17):9612. doi: 10.3390/ijms22179612

Figure 4.

Figure 4

Gene expression of pro-inflammatory markers in lung tissue and total nitric oxide in bronchoalveolar lavage. (A–F): mRNA expression by qPCR. Data are expressed mean ± SEM, ∆Ct correction was applied using GAPDH as a housekeeping gene and units are relative to the expression of control group. Sham and Sham + MTX n = 10; CLP and CLP + MTX n = 15. (G) Nitric oxide measured in bronchoalveolar lavage. Sham and Sham + MTX n = 5; CLP and CLP + MTX n = 6. * p < 0.05 vs. Sham group; # p < 0.05 vs. CLP group. ** p < 0.01; ***/### p < 0.001.