. 2021 Sep 5;22(17):9616. doi: 10.3390/ijms22179616

Table 2.

Antibodies and protocols used for immunoblotting.

Target	Primary Antibody	Incubation	Secondary Antibody
AMPK α p-T172	CST 2531	Overnight	GAR CST 7074
AMPK α p-T172	1:1000	4 °C	1:5000
AMPK α	CST 2532	Overnight	GAR CST 7074
AMPK α	1:1000	4 °C	1:5000
AMPK β1/β2	CST 4150	Overnight	GAR CST 7074
AMPK β1/β2	1:1000	4 °C	1:5000
ACC p-S79	CST 11818	Overnight	GAR CST 7074
ACC p-S79	1:1000	4 °C	1:5000
ACC	CST 3662	Overnight	GAR CST 7074
ACC	1:1000	4 °C	1:5000
Akt p-S473	CST 9271	1.5 h	GAR CST 7074
Akt p-S473	1:1000	Room temperature	1:5000
Akt	CST 4691	Overnight	GAR CST 7074
Akt	1:1000	4 °C	1:5000
AS160 p-T642	CST 4288	Overnight	GAR CST 7074
AS160 p-T642	1:1000	4 °C	1:5000
AS160	Abcam ab24469	Overnight	GAR CST 7074
AS160	1:1000	4 °C	1:5000
GLUT4	CST 2213	Overnight	GAM CST 7076
GLUT4	1:1000	4 °C	1:2000
GS p-S641	CST 47043	Overnight	GAR CST 7074
GS p-S641	1:1000	4 °C	1:5000
GS	CST 3886	Overnight	GAR CST 7074
GS	1:1000	4 °C	1:5000
Glycogenin1	Abcam ab11171	Overnight	GAR CST 7074
Glycogenin1	1:1000	4 °C	1:2000
GBE1	Abcam ab180596	Overnight	GAR CST 7074
GBE1	1:1000	4 °C	1:2000
GP	Antibody generated by B.E.K. laboratory	Overnight	GAR CST 7074
GP	1:1000	4 °C	1:2000
GDE	Antibody generated by B.E.K. laboratory	Overnight	GAR CST 7074
GDE	1:1000	4 °C	1:2000