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. 2021 Sep 5;22(17):9614. doi: 10.3390/ijms22179614

Figure 2.

Figure 2

Inhibition of A-site binding kinetics. Time courses of BPY-labelled ribosomal complex (0.1 µM) interaction with ternary complex (1 μM) containing EF-Tu from E. coli (black trace) or T. thermophilus (red trace) compared to the reaction, inhibited by 30 µM tetracycline with EF-Tu from E. coli (grey trace) or T. thermophilus (pink trace), 150 µM kirromycin with EF-Tu from E. coli (green trace), a modified variant of EF-Tu His84Ala from E. coli (violet trace) or buffer TAKM7 (blue trace). Each time course reflects the average of 5 to 7 technical replicates. S.e.m. associated with the kinetics were calculated by the GraphPad Prism software.