Skip to main content
. 2021 Sep 5;22(17):9614. doi: 10.3390/ijms22179614

Table 1.

Temperature dependence of rate constants of tRNA translocation.

E. coli T. thermophilus
T, °C kelbow, s−1 kcca, s−1 kelbow, s−1 kcca, s−1
15 2.1 4.9 1.9 2.3
20 5.2 7.7 5.7 4.7
25 8.7 11.9 8.9 6.9
30 15.7 18.2 15.5 8.9
37 31.7 26.1 31.5 19.0
42 52.2 39.2 55.8 28.7
50 83.0 66.1 111.0 62.1

Rate constants determined at 5 µM EF-G; Standard error of the mean did not exceed ±5%; kelbow—rate constant of translocation detected by the label at the elbow region of tRNA; kcca—rate constant of translocation detected by the label at CCA-end of tRNA.