Table 2.
Influence of antibiotics on tRNA translocation.
| E. coli | T. thermophilus | |||||
|---|---|---|---|---|---|---|
| A/b | k1 elbow, s−1 | k2 elbow, s−1 | kcca, s−1 | k1 elbow, s−1 | k2 elbow, s−1 | kcca, s−1 |
| No a/b | 35 | 1 | 23 | 33 | 0.6 | 14 |
| Str | 8 | 1 | 15 | 10 | 0.6 | 7 |
| Vio | - | - | 47 | 0.004 | - | 22 |
| Hyg B | 0.02 | - | 33 | 0.05 | - | 18 |
| Spc | 53 | 0.07 | 44 | 33 | 0.03 | 27 |
Rate constants determined at 5 µM EF-G. No influence of antibiotics on k2 of CCA movement was detected. Standard error of the mean did not exceed ±8%. A/b—antibiotic; Str—streptomycin; Vio—viomycin; Hyg B—hygromycin B; Spc—spectinomycin.