Figure 2. ZEB1 promotes Th1 and Th17 and inhibits Th2 differentiation in both humans and mice.

(A) Schematic for testing the role of ZEB1 in mouse Th cell in vitro differentiation.

(B–E) Flow cytometry evaluating Th cell differentiation of CD4⁺ naive T cells from WT or CD4^CreZEB1^L/L mice.

(F) Schematic of siRNA nucleofection and differentiation of naive CD4⁺ T cells under different polarizing conditions.

(G) Flow cytometry evaluating Th cell differentiation of human CD4⁺ naive T cells nucleofected with siZEB1 or siCTL at days 3, 5, and 7, respectively; n = 3 human donors.

(H–K) Flow cytometry evaluating Th cell differentiation of human CD4⁺ naive T cells nucleofected with siZEB1 or siCTL at the temporal peak of differentiation conditions.

Symbols in (B)–(E) represent individual mice. Symbols in (G)–(K) represent individual donors. Data in (B)–(E) are expressed as mean ± SEM. All statistical differences in (B)–(E) were tested using unpaired Student’s t test (two-tailed). All statistical differences in (G)–(K) were tested using paired Student’s t test (two-tailed). NS, not significant; *p < 0.05, **p < 0.01.